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SUPT16H Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SUPT16H.

  • WB - SUPT16H Antibody (monoclonal) (M01) AT4106a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.62 KDa) .
  • WB - SUPT16H Antibody (monoclonal) (M01) AT4106a
    SUPT16H monoclonal antibody (M01), clone 1D12 Western Blot analysis of SUPT16H expression in Hela S3 NE ( (Cat # AT4106a )
  • E - SUPT16H Antibody (monoclonal) (M01) AT4106a
    Detection limit for recombinant GST tagged SUPT16H is 0.03 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y5B9
Other Accession NM_007192
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D12
Calculated MW 119914 Da
Additional Information
Gene ID 11198
Other Names FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16, SUPT16H, FACT140, FACTP140
Target/Specificity SUPT16H (NP_009123, 608 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSUPT16H Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit.


Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Heo K, et al. Mol Cell, 2008 Apr 11. PMID 18406329.Histone H2A monoubiquitination represses transcription by inhibiting RNA polymerase II transcriptional elongation. Zhou W, et al. Mol Cell, 2008 Jan 18. PMID 18206970.Distinct class of putative non-conserved promoters in humans: comparative studies of alternative promoters of human and mouse genes. Tsuritani K, et al. Genome Res, 2007 Jul. PMID 17567985.Human SSRP1 has Spt16-dependent and -independent roles in gene transcription. Li Y, et al. J Biol Chem, 2007 Mar 9. PMID 17209051.

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$ 315.00
Cat# AT4106a
(40 western blots)
Availability: 7-10 days
Bulk Size
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