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>   home   >   Products   >   Primary Antibodies   >   TAF12 Antibody (monoclonal) (M11)   

TAF12 Antibody (monoclonal) (M11)

Mouse monoclonal antibody raised against a full-length recombinant TAF12.

  • WB - TAF12 Antibody (monoclonal) (M11) AT4142a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (43.45 KDa) .
  • WB - TAF12 Antibody (monoclonal) (M11) AT4142a
    TAF12 monoclonal antibody (M11), clone 1E10. Western Blot analysis of TAF12 expression in Hela S3 NE ( (Cat # AT4142a )
  • IF - TAF12 Antibody (monoclonal) (M11) AT4142a
    Immunofluorescence of monoclonal antibody to TAF12 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - TAF12 Antibody (monoclonal) (M11) AT4142a
    Detection limit for recombinant GST tagged TAF12 is 0.3 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16514
Other Accession BC011986
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1E11
Calculated MW 17924 Da
Additional Information
Gene ID 6883
Other Names Transcription initiation factor TFIID subunit 12, Transcription initiation factor TFIID 20/15 kDa subunits, TAFII-20/TAFII-15, TAFII20/TAFII15, TAF12, TAF15, TAF2J, TAFII20
Target/Specificity TAF12 (AAH11986, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTAF12 Antibody (monoclonal) (M11) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.


TAF4/4b x TAF12 displays a unique mode of DNA binding and is required for core promoter function of a subset of genes. Gazit K, et al. J Biol Chem, 2009 Sep 25. PMID 19635797.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Identification of novel functional TBP-binding sites and general factor repertoires. Denissov S, et al. EMBO J, 2007 Feb 21. PMID 17268553.The DNA sequence and biological annotation of human chromosome 1. Gregory SG, et al. Nature, 2006 May 18. PMID 16710414.Glutamine-expanded ataxin-7 alters TFTC/STAGA recruitment and chromatin structure leading to photoreceptor dysfunction. Helmlinger D, et al. PLoS Biol, 2006 Mar. PMID 16494529.

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$ 315.00
Cat# AT4142a
(40 western blots)
Availability: 7-10 days
Bulk Size
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