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TIPIN Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant TIPIN.

     
  • IF - TIPIN Antibody (monoclonal) (M01) AT4247a
    Immunofluorescence of monoclonal antibody to TIPIN on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - TIPIN Antibody (monoclonal) (M01) AT4247a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (58.85 KDa) .
    detail
  • WB - TIPIN Antibody (monoclonal) (M01) AT4247a
    Western Blot analysis of TIPIN expression in transfected 293T cell line by TIPIN monoclonal antibody (M01), clone 1G11.

    Lane 1: TIPIN transfected lysate(34.496 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - TIPIN Antibody (monoclonal) (M01) AT4247a
    Detection limit for recombinant GST tagged TIPIN is 0.03 ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9BVW5
Other Accession BC000870
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1G11
Calculated MW 34555 Da
Additional Information
Gene ID 54962
Other Names TIMELESS-interacting protein, TIPIN
Target/Specificity TIPIN (AAH00870, 1 a.a. ~ 301 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTIPIN Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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References

Tipin-replication protein A interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress. Kemp MG, et al. J Biol Chem, 2010 May 28. PMID 20233725.Systematic analysis of circadian genes in a population-based sample reveals association of TIMELESS with depression and sleep disturbance. Utge SJ, et al. PLoS One, 2010 Feb 18. PMID 20174623.Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. Leman AR, et al. J Cell Sci, 2010 Mar 1. PMID 20124417.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Unsal-Ka?maz K, et al. Mol Cell Biol, 2007 Apr. PMID 17296725.

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$ 350.00
Cat# AT4247a
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