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TNP1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant TNP1.

     
  • WB - TNP1 Antibody (monoclonal) (M01) AT4294a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (31.79 KDa) .
    detail
  • IHC - TNP1 Antibody (monoclonal) (M01) AT4294a
    Immunoperoxidase of monoclonal antibody to TNP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
    detail
  • E - TNP1 Antibody (monoclonal) (M01) AT4294a
    Detection limit for recombinant GST tagged TNP1 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P09430
Other Accession BC029516
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b kappa
Clone Names 1B5
Calculated MW 6424 Da
Additional Information
Gene ID 7141
Other Names Spermatid nuclear transition protein 1, STP-1, TP-1, TNP1
Target/Specificity TNP1 (AAH29516.1, 1 a.a. ~ 55 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTNP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines (see PRM1, MIM 182880; PRM2, MIM 182890) (Luerssen et al., 1990 [PubMed 2249851]).

References

The TNP1 haplotype - GCG is associated with azoospermia. Khattri A, et al. Int J Androl, 2010 May 26. PMID 20522125.Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent. Aston KI, et al. Hum Reprod, 2010 Jun. PMID 20378615.Genome-wide association study reveals multiple loci associated with primary tooth development during infancy. Pillas D, et al. PLoS Genet, 2010 Feb 26. PMID 20195514.A multistage genome-wide association study in breast cancer identifies two new risk alleles at 1p11.2 and 14q24.1 (RAD51L1). Thomas G, et al. Nat Genet, 2009 May. PMID 19330030.Spermiogenesis defects in human: detection of transition proteins in semen from some infertile men. Becker S, et al. Andrologia, 2008 Aug. PMID 18727729.

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$ 350.00
Cat# AT4294a
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Availability: 7-10 days
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