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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   TOP1 Antibody (monoclonal) (M01)   

TOP1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TOP1.

     
  • WB - TOP1 Antibody (monoclonal) (M01) AT4304a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33.88 KDa) .
  • WB - TOP1 Antibody (monoclonal) (M01) AT4304a
    TOP1 monoclonal antibody (M01), clone 1A1. Western Blot analysis of TOP1 expression in human ovarian cancer.
  • WB - TOP1 Antibody (monoclonal) (M01) AT4304a
    TOP1 monoclonal antibody (M01), clone 1A1. Western Blot analysis of TOP1 expression in HeLa.
  • WB - TOP1 Antibody (monoclonal) (M01) AT4304a
    TOP1 monoclonal antibody (M01), clone 1A1. Western Blot analysis of TOP1 expression in JAR.
  • IHC - TOP1 Antibody (monoclonal) (M01) AT4304a
    Immunoperoxidase of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. [antibody concentration 3 ug/ml]
  • IHC - TOP1 Antibody (monoclonal) (M01) AT4304a
    Immunoperoxidase of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1~10 ug/ml]
  • E - TOP1 Antibody (monoclonal) (M01) AT4304a
    Detection limit for recombinant GST tagged TOP1 is approximately 0.3ng/ml as a capture antibody.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P11387
Other Accession NM_003286
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1A1
Calculated MW 90726 Da
Additional Information
Gene ID 7150
Other Names DNA topoisomerase 1, DNA topoisomerase I, TOP1
Target/Specificity TOP1 (NP_003277, 692 a.a. ~ 765 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTOP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

References

1.AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination.Kobayashi M, Aida M, Nagaoka H, Begum NA, Kitawaki Y, Nakata M, Stanlie A, Doi T, Kato L, Okazaki IM, Shinkura R, Muramatsu M, Kinoshita K, Honjo T.Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22375-80. Epub 2009 Dec 11.

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$ 315.00
Cat# AT4304a
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