TRIM32 Antibody (monoclonal) (M09)
Mouse monoclonal antibody raised against a partial recombinant TRIM32.
|Application ||WB, IF, E|
|Calculated MW||71989 Da|
|Other Names||E3 ubiquitin-protein ligase TRIM32, 632-, 72 kDa Tat-interacting protein, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A|
|Target/Specificity||TRIM32 (NP_036342, 105 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||TRIM32 Antibody (monoclonal) (M09) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
1.Nuclear factor kappa B signaling initiates early differentiation of neural stem cells.Zhang Y, Liu J, Yao S, Li F, Xin L, Lai M, Bracchi-Ricard V, Xu H, Yen W, Meng W, Liu S, Yang L, Karmally S, Liu J, Zhu H, Gordon J, Khalili K, Srinivasan S, Bethea JR, Mo X, Hu W.Stem Cells. 2012 Mar;30(3):510-24. doi: 10.1002/stem.1006.2.TRIM32 Regulates Skeletal Muscle Stem Cell Differentiation and Is Necessary for Normal Adult Muscle Regeneration.Nicklas S, Otto A, Wu X, Miller P, Stelzer S, Wen Y, Kuang S, Wrogemann K, Patel K, Ding H, Schwamborn JC.PLoS One. 2012;7(1):e30445. Epub 2012 Jan 27.3.TRIM32 promotes neural differentiation through retinoic acid receptor-mediated transcription.Sato T, Okumura F, Kano S, Kondo T, Ariga T, Hatakeyama S.J Cell Sci. 2011 Oct 15;124(Pt 20):3492-502. Epub 2011 Oct 7.4.The TRIM-NHL Protein TRIM32 Activates MicroRNAs and Prevents Self-Renewal in Mouse Neural Progenitors.Schwamborn JC, Berezikov E, Knoblich JA.Cell. 2009 Mar 6;136(5):913-25.
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