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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   TRIM32 Antibody (monoclonal) (M09)   

TRIM32 Antibody (monoclonal) (M09)

Mouse monoclonal antibody raised against a partial recombinant TRIM32.

  • WB - TRIM32 Antibody (monoclonal) (M09) AT4350a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - TRIM32 Antibody (monoclonal) (M09) AT4350a
    Western Blot analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5.

    Lane 1: TRIM32 transfected lysate(72 KDa).
    Lane 2: Non-transfected lysate.
  • IF - TRIM32 Antibody (monoclonal) (M09) AT4350a
    Immunofluorescence of monoclonal antibody to TRIM32 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - TRIM32 Antibody (monoclonal) (M09) AT4350a
    Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.
  • WB - TRIM32 Antibody (monoclonal) (M09) AT4350a
    Western blot analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi ( (Cat # AT4350a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13049
Other Accession NM_012210
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2E6
Calculated MW 71989 Da
Additional Information
Gene ID 22954
Other Names E3 ubiquitin-protein ligase TRIM32, 632-, 72 kDa Tat-interacting protein, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A
Target/Specificity TRIM32 (NP_036342, 105 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTRIM32 Antibody (monoclonal) (M09) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.


1.Nuclear factor kappa B signaling initiates early differentiation of neural stem cells.Zhang Y, Liu J, Yao S, Li F, Xin L, Lai M, Bracchi-Ricard V, Xu H, Yen W, Meng W, Liu S, Yang L, Karmally S, Liu J, Zhu H, Gordon J, Khalili K, Srinivasan S, Bethea JR, Mo X, Hu W.Stem Cells. 2012 Mar;30(3):510-24. doi: 10.1002/stem.1006.2.TRIM32 Regulates Skeletal Muscle Stem Cell Differentiation and Is Necessary for Normal Adult Muscle Regeneration.Nicklas S, Otto A, Wu X, Miller P, Stelzer S, Wen Y, Kuang S, Wrogemann K, Patel K, Ding H, Schwamborn JC.PLoS One. 2012;7(1):e30445. Epub 2012 Jan 27.3.TRIM32 promotes neural differentiation through retinoic acid receptor-mediated transcription.Sato T, Okumura F, Kano S, Kondo T, Ariga T, Hatakeyama S.J Cell Sci. 2011 Oct 15;124(Pt 20):3492-502. Epub 2011 Oct 7.4.The TRIM-NHL Protein TRIM32 Activates MicroRNAs and Prevents Self-Renewal in Mouse Neural Progenitors.Schwamborn JC, Berezikov E, Knoblich JA.Cell. 2009 Mar 6;136(5):913-25.

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$ 315.00
Cat# AT4350a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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