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TWIST1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TWIST1.

     
  • IP - TWIST1 Antibody (monoclonal) (M01) AT4410a
    Immunoprecipitation of TWIST1 transfected lysate using anti-TWIST1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TWIST1 MaxPab rabbit polyclonal antibody.
    detail
  • WB - TWIST1 Antibody (monoclonal) (M01) AT4410a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.07 KDa) .
    detail
  • WB - TWIST1 Antibody (monoclonal) (M01) AT4410a
    TWIST1 monoclonal antibody (M01), clone 3E11. Western Blot analysis of TWIST1 expression in rat brain.
    detail
  • WB - TWIST1 Antibody (monoclonal) (M01) AT4410a
    Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M01), clone 3E11.

    Lane 1: TWIST1 transfected lysate(21 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - TWIST1 Antibody (monoclonal) (M01) AT4410a
    Detection limit for recombinant GST tagged TWIST1 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession Q15672
Other Accession NM_000474
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3E12
Calculated MW 20954 Da
Additional Information
Gene ID 7291
Other Names Twist-related protein 1, Class A basic helix-loop-helix protein 38, bHLHa38, H-twist, TWIST1, BHLHA38, TWIST
Target/Specificity TWIST1 (NP_000465, 100 a.a. ~ 202 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTWIST1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

References

1.Cellular migration and invasion uncoupled: Increased migration is not an inexorable consequence of EMT.Schaeffer D, Somarelli JA, Hanna G, Palmer GM, Garcia-Blanco MAMol Cell Biol. 2014 Jul 7. pii: MCB.00694-14.2.EMT and induction of miR-21 mediate metastasis development in Trp53-deficient tumours.Bornachea O, Santos M, Martinez-Cruz AB, Garcia-Escudero R, Duenas M, Costa C, Segrelles C, Lorz C, Buitrago A, Saiz-Ladera C, Agirre X, Grande T, Paradela B, Maraver A, Ariza JM, Prosper F, Serrano M, Sanchez-Cespedes M, Paramio JM.Sci Rep. 2012;2:434. Epub 2012 May 31.3.Conversion of Helicobacter pylori CagA from senescence inducer to oncogenic driver through polarity-dependent regulation of p21.Saito Y, Murata-Kamiya N, Hirayama T, Ohba Y, Hatakeyama M.J Exp Med. 2010 Sep 27;207(10):2157-74. Epub 2010 Sep 20.

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$ 350.00
Cat# AT4410a
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Availability: 7-10 days
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