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UBE2M Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant UBE2M.

     
  • IF - UBE2M Antibody (monoclonal) (M01) AT4440a
    Immunofluorescence of monoclonal antibody to UBE2M on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - UBE2M Antibody (monoclonal) (M01) AT4440a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (45.87 KDa) .
    detail
  • WB - UBE2M Antibody (monoclonal) (M01) AT4440a
    UBE2M monoclonal antibody (M01), clone 3C4 Western Blot analysis of UBE2M expression in Jurkat ( (Cat # AT4440a )
    detail
  • E - UBE2M Antibody (monoclonal) (M01) AT4440a
    Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P61081
Other Accession BC058924
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3C4
Calculated MW 20900 Da
Additional Information
Gene ID 9040
Other Names NEDD8-conjugating enzyme Ubc12, 632-, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBE2M, UBC12
Target/Specificity UBE2M (AAH58924, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUBE2M Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

References

1.Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway.Miller BW, Lau G, Grouios C, Mollica E, Barrios-Rodiles M, Liu Y, Datti A, Morris Q, Wrana JL, Attisano L.Molecular Systems Biology 5; Article number 315; doi:10.1038 /msb.2009.72

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$ 350.00
Cat# AT4440a
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