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UBE2M Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant UBE2M.

  • WB - UBE2M Antibody (monoclonal) (M01) AT4440a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (45.87 KDa) .
  • WB - UBE2M Antibody (monoclonal) (M01) AT4440a
    UBE2M monoclonal antibody (M01), clone 3C4 Western Blot analysis of UBE2M expression in Jurkat ( (Cat # AT4440a )
  • IF - UBE2M Antibody (monoclonal) (M01) AT4440a
    Immunofluorescence of monoclonal antibody to UBE2M on HeLa cell. [antibody concentration 10 ug/ml]
  • E - UBE2M Antibody (monoclonal) (M01) AT4440a
    Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P61081
Other Accession BC058924
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3C4
Calculated MW 20900 Da
Additional Information
Gene ID 9040
Other Names NEDD8-conjugating enzyme Ubc12, 632-, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBE2M, UBC12
Target/Specificity UBE2M (AAH58924, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUBE2M Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.


1.Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway.Miller BW, Lau G, Grouios C, Mollica E, Barrios-Rodiles M, Liu Y, Datti A, Morris Q, Wrana JL, Attisano L.Molecular Systems Biology 5; Article number 315; doi:10.1038 /msb.2009.72

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$ 315.00
Cat# AT4440a
(40 western blots)
Availability: 7-10 days
Bulk Size
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