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UBR2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant UBR2.

  • WB - UBR2 Antibody (monoclonal) (M01) AT4451a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - UBR2 Antibody (monoclonal) (M01) AT4451a
    Western Blot analysis of UBR2 expression in transfected 293T cell line by UBR2 monoclonal antibody (M01), clone 4G4.

    Lane 1: UBR2 transfected lysate(50 KDa).
    Lane 2: Non-transfected lysate.
  • IF - UBR2 Antibody (monoclonal) (M01) AT4451a
    Immunofluorescence of monoclonal antibody to UBR2 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - UBR2 Antibody (monoclonal) (M01) AT4451a
    Detection limit for recombinant GST tagged UBR2 is approximately 0.3ng/ml as a capture antibody.
  • WB - UBR2 Antibody (monoclonal) (M01) AT4451a
    Western blot analysis of UBR2 over-expressed 293 cell line, cotransfected with UBR2 Validated Chimera RNAi ( (Cat # AT4451a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IWV8
Other Accession NM_015255
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4G4
Calculated MW 200538 Da
Additional Information
Gene ID 23304
Other Names E3 ubiquitin-protein ligase UBR2, 632-, N-recognin-2, Ubiquitin-protein ligase E3-alpha-2, Ubiquitin-protein ligase E3-alpha-II, UBR2, C6orf133, KIAA0349
Target/Specificity UBR2 (NP_056070, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUBR2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway. Kume K, et al. Genes Cells, 2010 Apr 1. PMID 20298436.Identification of a novel antibody associated with autoimmune pancreatitis. Frulloni L, et al. N Engl J Med, 2009 Nov 26. PMID 19940298.Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Jeronimo C, et al. Mol Cell, 2007 Jul 20. PMID 17643375.Deficiency of UBR1, a ubiquitin ligase of the N-end rule pathway, causes pancreatic dysfunction, malformations and mental retardation (Johanson-Blizzard syndrome). Zenker M, et al. Nat Genet, 2005 Dec. PMID 16311597.

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$ 315.00
Cat# AT4451a
(40 western blots)
Availability: 7-10 days
Bulk Size
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