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VAPA Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full-length recombinant VAPA.

     
  • IF - VAPA Antibody (monoclonal) (M01) AT4501a
    Immunofluorescence of monoclonal antibody to VAPA on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - VAPA Antibody (monoclonal) (M01) AT4501a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (52.36 KDa) .
    detail
  • WB - VAPA Antibody (monoclonal) (M01) AT4501a
    VAPA monoclonal antibody (M01), clone 4C12. Western Blot analysis of VAPA expression in human placenta.
    detail
  • WB - VAPA Antibody (monoclonal) (M01) AT4501a
    VAPA monoclonal antibody (M01), clone 4C12. Western Blot analysis of VAPA expression in K-562.
    detail
  • WB - VAPA Antibody (monoclonal) (M01) AT4501a
    Western Blot analysis of VAPA expression in transfected 293T cell line by VAPA monoclonal antibody (M01), clone 4C12.

    Lane 1: VAPA transfected lysate(27.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9P0L0
Other Accession BC002992
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4C12
Calculated MW 27893 Da
Additional Information
Gene ID 9218
Other Names Vesicle-associated membrane protein-associated protein A, VAMP-A, VAMP-associated protein A, VAP-A, 33 kDa VAMP-associated protein, VAP-33, VAPA, VAP33
Target/Specificity VAPA (AAH02992, 1 a.a. ~ 242 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsVAPA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

References

Electrostatic interaction between oxysterol-binding protein and VAMP-associated protein A revealed by NMR and mutagenesis studies. Furuita K, et al. J Biol Chem, 2010 Apr 23. PMID 20178991.The glycolipid transfer protein interacts with the vesicle-associated membrane protein-associated protein VAP-A. Tuuf J, et al. Biochem Biophys Res Commun, 2009 Oct 16. PMID 19665998.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. Saita S, et al. J Biol Chem, 2009 May 15. PMID 19289470.FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation. Prosser DC, et al. J Cell Sci, 2008 Sep 15. PMID 18713837.

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$ 350.00
Cat# AT4501a
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Availability: 7-10 days
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