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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VRK2 Antibody (monoclonal) (M01)   

VRK2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant VRK2.

  • WB - VRK2 Antibody (monoclonal) (M01) AT4522a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - VRK2 Antibody (monoclonal) (M01) AT4522a
    VRK2 monoclonal antibody (M01), clone 3B10. Western Blot analysis of VRK2 expression in U-2 OS ( (Cat # AT4522a )
  • WB - VRK2 Antibody (monoclonal) (M01) AT4522a
    VRK2 monoclonal antibody (M01), clone 3B10 Western Blot analysis of VRK2 expression in K-562 ( (Cat # AT4522a )
  • WB - VRK2 Antibody (monoclonal) (M01) AT4522a
    Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody (M01), clone 3B10.

    Lane 1: VRK2 transfected lysate(58.1 KDa).
    Lane 2: Non-transfected lysate.
  • IF - VRK2 Antibody (monoclonal) (M01) AT4522a
    Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - VRK2 Antibody (monoclonal) (M01) AT4522a
    Detection limit for recombinant GST tagged VRK2 is approximately 1ng/ml as a capture antibody.
  • WB - VRK2 Antibody (monoclonal) (M01) AT4522a
    Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi ( (Cat # AT4522a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86Y07
Other Accession BC027854
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3B10
Calculated MW 58141 Da
Additional Information
Gene ID 7444
Other Names Serine/threonine-protein kinase VRK2, Vaccinia-related kinase 2, VRK2
Target/Specificity VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsVRK2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. Multiple alternatively spliced transcript variants have been found for this gene.


Common variants conferring risk of schizophrenia. Stefansson H, et al. Nature, 2009 Aug 6. PMID 19571808.Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Sanz-Garc?a M, et al. Mol Cell Proteomics, 2008 Nov. PMID 18617507.The C/H3 domain of p300 is required to protect VRK1 and VRK2 from their downregulation induced by p53. Valbuena A, et al. PLoS One, 2008 Jul 9. PMID 18612383.Modulation of interleukin-1 transcriptional response by the interaction between VRK2 and the JIP1 scaffold protein. Blanco S, et al. PLoS One, 2008 Feb 20. PMID 18286207.Vaccinia-related kinase 2 modulates the stress response to hypoxia mediated by TAK1. Blanco S, et al. Mol Cell Biol, 2007 Oct. PMID 17709393.

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$ 315.00
Cat# AT4522a
(40 western blots)
Availability: 7-10 days
Bulk Size
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