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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   WDR77 Antibody (monoclonal) (M01)   

WDR77 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant WDR77.

  • WB - WDR77 Antibody (monoclonal) (M01) AT4536a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (63.36 KDa) .
  • WB - WDR77 Antibody (monoclonal) (M01) AT4536a
    WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( (Cat # AT4536a )
  • WB - WDR77 Antibody (monoclonal) (M01) AT4536a
    Western Blot analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.

    Lane 1: WDR77 transfected lysate(36.7 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - WDR77 Antibody (monoclonal) (M01) AT4536a
    Immunoperoxidase of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
  • E - WDR77 Antibody (monoclonal) (M01) AT4536a
    Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BQA1
Other Accession BC001679
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3F10
Calculated MW 36724 Da
Additional Information
Gene ID 79084
Other Names Methylosome protein 50, MEP-50, Androgen receptor cofactor p44, WD repeat-containing protein 77, p44/Mep50, WDR77, MEP50, WD45
Target/Specificity WDR77 (AAH01679, 1 a.a. ~ 342 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsWDR77 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).


1.PRMT5 Is Upregulated in Malignant and Metastatic Melanoma and Regulates Expression of MITF and p27(Kip1.).Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sif S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GBPLoS One. 2013 Sep 30;8(9):e74710. doi: 10.1371/journal.pone.0074710.

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$ 315.00
Cat# AT4536a
(40 western blots)
Availability: 7-10 days
Bulk Size
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