WWP1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant WWP1.
|Application ||WB, IHC, IF, IP, E|
|Calculated MW||105202 Da|
|Other Names||NEDD4-like E3 ubiquitin-protein ligase WWP1, 632-, Atrophin-1-interacting protein 5, AIP5, TGIF-interacting ubiquitin ligase 1, Tiul1, WW domain-containing protein 1, WWP1|
|Target/Specificity||WWP1 (NP_008944, 152 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||WWP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.
1.Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53.Cheng Q, Cao X, Yuan F, Li G, Tong TBiochem Biophys Res Commun. 2014 May 1. pii: S0006-291X(14)00780-3. doi: 10.1016/j.bbrc.2014.04.117.2.WWP1 delays cellular senescence by promoting p27Kip1 degradation in human diploid fibroblasts.Cao X, Xue L, Han L, Ma L, Chen T, Tong T.J Biol Chem. 2011 Jul 27. [Epub ahead of print]3.The E3 ubiquitin ligase WWP1 regulates ?GNp63-dependent transcription through Lys63 linkages.Peschiaroli A, Scialpi F, Bernassola F, Sherbini el SE, Melino G.Biochem Biophys Res Commun. 2010 Nov 12;402(2):425-30. Epub 2010 Oct 15.4.Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.Edwards TL, Clowes VE, Tsang HT, Connell JW, Sanderson CM, Luzio JP, Reid E.Biochem J. 2009 Sep 14;423(1):31-9.
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