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YWHAG Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant YWHAG.

     
  • WB - YWHAG Antibody (monoclonal) (M02) AT4560a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - YWHAG Antibody (monoclonal) (M02) AT4560a
    YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in PC-12 ( (Cat # AT4560a )
    detail
  • WB - YWHAG Antibody (monoclonal) (M02) AT4560a
    YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in IMR-32 ( (Cat # AT4560a )
    detail
  • WB - YWHAG Antibody (monoclonal) (M02) AT4560a
    YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in Raw 264.7 ( (Cat # AT4560a )
    detail
  • WB - YWHAG Antibody (monoclonal) (M02) AT4560a
    YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in NIH/3T3 ( (Cat # AT4560a )
    detail
  • WB - YWHAG Antibody (monoclonal) (M02) AT4560a
    YWHAG monoclonal antibody (M02), clone 6A10 Western Blot analysis of YWHAG expression in K-562 ( (Cat # AT4560a )
    detail
  • E - YWHAG Antibody (monoclonal) (M02) AT4560a
    Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P61981
Other Accession NM_012479
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 6A10
Calculated MW 28303 Da
Additional Information
Gene ID 7532
Other Names 14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma, N-terminally processed, YWHAG
Target/Specificity YWHAG (NP_036611, 70 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsYWHAG Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

References

Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.The expression of seven 14-3-3 isoforms in human meningioma. Liu Y, et al. Brain Res, 2010 Jun 8. PMID 20388496.Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070.Zebrafish gene knockdowns imply roles for human YWHAG in infantile spasms and cardiomegaly. Komoike Y, et al. Genesis, 2010 Apr. PMID 20146355.Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase, and negatively charged membranes. Halskau ? Jr, et al. J Biol Chem, 2009 Nov 20. PMID 19801645.

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$ 350.00
Cat# AT4560a
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Availability: 7-10 days
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