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ZW10 Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant ZW10.

  • WB - ZW10 Antibody (monoclonal) (M05) AT4649a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - ZW10 Antibody (monoclonal) (M05) AT4649a
    Western Blot analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5.

    Lane 1: ZW10 transfected lysate(88.8 KDa).
    Lane 2: Non-transfected lysate.
  • E - ZW10 Antibody (monoclonal) (M05) AT4649a
    Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.
  • WB - ZW10 Antibody (monoclonal) (M05) AT4649a
    Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi ( (Cat # AT4649a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43264
Other Accession NM_004724
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2E6
Calculated MW 88829 Da
Additional Information
Gene ID 9183
Other Names Centromere/kinetochore protein zw10 homolog, ZW10
Target/Specificity ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsZW10 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.


Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. Chan YW, et al. J Cell Biol, 2009 Jun 1. PMID 19468067.Identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18 complex implicated in Golgi-to-endoplasmic reticulum retrograde transport. Aoki T, et al. Mol Biol Cell, 2009 Jun. PMID 19369418.N-terminal region of ZW10 serves not only as a determinant for localization but also as a link with dynein function. Inoue M, et al. Genes Cells, 2008 Aug. PMID 18782227.Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint. Famulski JK, et al. J Cell Biol, 2008 Feb 11. PMID 18268100.Aurora B kinase-dependent recruitment of hZW10 and hROD to tensionless kinetochores. Famulski JK, et al. Curr Biol, 2007 Dec 18. PMID 18065224.

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$ 315.00
Cat# AT4649a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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