APC Anti-Mouse CD3 (17A2) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC |
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Isotype | Rat IgG2b, κ |
Concentration | 0.2 mg/mL |
Reactivity | Mouse |
Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
Host | Rat |
Gene ID | 12502 |
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Gene Name | Cd3g |
Alternative Name(s) | CD3 epsilon |
Format | APC |
Application Notes | This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
Storage Conditions | 2-8°C protected from light |
Joetham A, Ohnishi H, Okamoto M, Takeda K, Schedel M, Domenico J, Dakhama A, and Gelfand EW. 2012. J. Biol. Chem. 287:17100-17108. (in vitro activation)
Kasahara S and Clark, EA. 2012. J. Leukoc. Biol. 91:437-448. (in vitro activation)
Xiao J, Julianty A, Wen J, Smith SV, Park PW, Ford ML, Haller CA, and Chaikof EL. 2012. Arterioscler. Thromb. Vasc. Biol. 32:386-396. (in vivo T cell depletion)
Bas A, Swamy M, Abeler-Dorner L, Williams G, Pang DJ, Barbee SD, and Hayday AC. 2011 Proc. Natl. Acad. Sci. 108: 4376-4381. (Immunohistochemistry - Paraffin embedded sections)
Miescher GC, Schreyer M, and MacDonald HR. 1989. Immunol. Lett. 23: 113-118. (Origination of clone 17A2, Functional Assay, Immunohistochemistry, Immunoprecipitation)
Provided below are standard protocols that you may find useful for product applications.
Background
The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.
The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. Journal of Biological Chemistry. 287: 13324-13335).
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