APC Anti-Human CD178 (Fas ligand) (NOK-1) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC |
---|---|
Isotype | Mouse IgG1, kappa |
Concentration | 5 uL (0.25ug)/test |
Reactivity | Human |
Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
Host | Mouse |
Gene ID | 356 |
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Gene Name | FASLG |
Alternative Name(s) | CD95L, Fas ligand, FasL |
Format | APC |
Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
Application Notes | This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. |
Storage Conditions | 2-8°C protected from light |
Tanaka M, Suda T, Takahashi T, and Nagata S. 1995. EMBO J. 14(6):1129-1135.
Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, and Yagita H. 1995. J Exp Med. 182(6):1777-1783.
Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S. 1997. J Exp Med. 186(12):2045-2050.
Ehrenschwender M, Wajant H. 2009. Adv Exp Med Biol. 647:64-93.
Provided below are standard protocols that you may find useful for product applications.
Background
The NOK-1 antibody reacts with human CD178 (Fas ligand) in both membrane bound and soluble forms. Fas ligand is a 40 kDa transmembrane glycoprotein, a member of the TNF family, and is expressed by activated T and NK cells, neutrophils, and monocytes. Interactions between CD178 (Fas ligand) and CD95 (Fas) induce a program of apoptosis and play a key role in immune regulation and homeostasis. The extracellular domain of human CD178 can be cleaved from the surface by matrix metalloproteinases (MMPs) resulting in a 26 kDa soluble protein.
References
Tanaka M, Suda T, Takahashi T, and Nagata S. 1995. EMBO J. 14(6):1129-1135. Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, and Yagita H. 1995. J Exp Med. 182(6):1777-1783. Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S. 1997. J Exp Med. 186(12):2045-2050. Ehrenschwender M, Wajant H. 2009. Adv Exp Med Biol. 647:64-93.
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