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FITC Anti-Mouse CD2 (RM2-5) Antibody

     
  • FC - FITC Anti-Mouse CD2 (RM2-5) Antibody ATB10374-U025
    C57Bl/6 splenocytes were stained with 0.25 ug FITC Anti-Mouse CD2 (ATB10374) (solid line) or 0.25 ug FITC Rat IgG2b (dashed line).
    detail
  • FC - FITC Anti-Mouse CD2 (RM2-5) Antibody ATB10374-U025
    C57Bl/6 splenocytes were stained with 0.125 ug FITC Anti-Mouse CD2 (RM2-5) manufactured by Tonbo Biosciences (left panel) or BioLegend (right panel).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Isotype Rat IgG2b, lambda
Concentration 0.5 mg/mL
Reactivity Mouse
Formulation 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Host Rat
Additional Information
Gene ID 12481
Gene Name Cd2
Alternative Name(s) LFA-2, Ly-37
Format FITC
Preparation This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
Application Notes This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.
Storage Conditions 2-8°C protected from light

Yagita H, Nakamura T, Karasuyama H, Okumura K. 1989. Proc. Natl. Acad. Sci. 86(2):645-9.

Nakamura T, Takahashi K, Fukazawa T, Koyanagi M, Yokoyama A, Kato H, Yagita H, Okumura K. 1990. J. Immunol. 145(11):3628-34.

Criado G, Feito MJ, Rojo JM. 1996. Eur. J. Immunol. 26(6):1228-34.

Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. 1997. Immunity. 6(3):245-55.

Background

The RM2-5 antibody reacts with mouse CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD48 in the mouse and is expressed on the cell surface of all mouse lymphocytes. CD2 mediates adhesion between cells and is involved in T cell activation.RM2-5 is reported to block CD2-mediated cell:cell adhesion.

References

Yagita H, Nakamura T, Karasuyama H, Okumura K. 1989. Proc. Natl. Acad. Sci. 86(2):645-9. Nakamura T, Takahashi K, Fukazawa T, Koyanagi M, Yokoyama A, Kato H, Yagita H, Okumura K. 1990. J. Immunol. 145(11):3628-34. Criado G, Feito MJ, Rojo JM. 1996. Eur. J. Immunol. 26(6):1228-34. Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. 1997. Immunity. 6(3):245-55.

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$ 175.00
Cat# ATB10374-U025
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