In Vivo Ready™ Anti-Mouse CD152 (CTLA-4) (UC10-4F10-11) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, FA |
---|---|
Isotype | Armenian Hamster IgG1, kappa |
Concentration | 2.0 mg/mL |
Reactivity | Mouse |
Formulation | 10 mM NaH2PO4, 150 mM NaCl, pH7.2 |
Host | Armenian Hamster |
Gene ID | 12477 |
---|---|
Gene Name | Ctla4 |
Alternative Name(s) | CTLA4 |
Format | In Vivo Ready™ |
Preparation | This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze. |
Application Notes | Tonbo Biosciences tests all of our antibodies by flow cytometry. Citations may be provided as a resource for additional applications that have not been validated by Tonbo Biosciences. |
Storage Conditions | 2-8°C |
Lischke T, Hegemann A, Gurka S, Van DV, Burmeister Y, Lam K-P, Kershaw O, Mollenkopf H-J, Mages HW, Hutloff A, and Kroczek RA. 2012. J. Immunol. 189: 234-244. (Flow Cytometry).
Tai X, Laethem FV, Pobezinsky L, Guinter T, Sharrow SO, Adams A, Granger L, Kruhlak M, Lindsten T, Thompson CB, Feigenbaum L, and Singer A. 2012. 119: 5155-5163. (Flow Cytometry).
Matheu MP, Su Y, Greenberg ML, Blanc CA, Parker I, Scott DW, and Calahan MD. 2012. 109: E1258-E1266. (in vitro blocking).
Provided below are standard protocols that you may find useful for product applications.
Background
The UC10-4F10-11 antibody is specific for mouse CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar, yet functionally disparate, to the T cell co-stimulatory molecule CD28. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells.CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be rapidly transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions. The UC10-4F10-11 antibody may be used for flow cytometric analysis of CTLA-4 expression.
References
Lischke T, Hegemann A, Gurka S, Van DV, Burmeister Y, Lam K-P, Kershaw O, Mollenkopf H-J, Mages HW, Hutloff A, and Kroczek RA. 2012. J. Immunol. 189: 234-244. (Flow Cytometry). Tai X, Laethem FV, Pobezinsky L, Guinter T, Sharrow SO, Adams A, Granger L, Kruhlak M, Lindsten T, Thompson CB, Feigenbaum L, and Singer A. 2012. 119: 5155-5163. (Flow Cytometry). Matheu MP, Su Y, Greenberg ML, Blanc CA, Parker I, Scott DW, and Calahan MD. 2012. 109: E1258-E1266. (in vitro blocking).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.