|Application ||IHC, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||49318 Da|
|Application & Usage||This anti-peptide antibody mixture was validated by immunohistochemistry (at a concentration of 2 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution) as the secondary reagent.|
|Other Names||Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX, DBCN, LISX|
|Format||Mixture of IgY fraction (10 mg/ml) and affinity-purified antibodies (50 ug/ml) in Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v).|
|Reconstitution & Storage||-20°C|
|Precautions||Doublecortin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.|
|Cellular Location||Cytoplasm. Cell projection. Note=Localizes at neurite tips.|
|Tissue Location||Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas|
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