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AK4 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P, WB |
|---|---|
| Primary Accession | P27144 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=25;M=25;Rat=25 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 205 |
|---|---|
| Antigen Region | 119-153 aa |
| Other Names | Adenylate kinase 4, mitochondrial, AK 4, Adenylate kinase 3-like, GTP:AMP phosphotransferase AK4, AK4, AK3, AK3L1 |
| Dilution | IF~~1:25 WB~~1:1000 IHC-P~~1:25 |
| Target/Specificity | This AK4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-153 amino acids from the Central region of human AK4. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AK4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AK4 {ECO:0000255|HAMAP-Rule:MF_03170} |
|---|---|
| Function | Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor (PubMed:19073142, PubMed:19766732, PubMed:23416111). Also displays broad nucleoside diphosphate kinase activity (PubMed:19073142, PubMed:19766732, PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435). |
| Cellular Location | Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03170, ECO:0000269|PubMed:11485571, ECO:0000269|PubMed:19766732, ECO:0000269|PubMed:26980435} |
| Tissue Location | Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain |
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Provided below are standard protocols that you may find useful for product applications.
Background
Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.
References
Xu G.,et al.Genomics 13:537-542(1992).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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