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HLA-B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HLA-B Antibody (N-term) AW5047-U400
    Western blot analysis of lysates from Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat. #AW5047). AW5047 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • FC - HLA-B Antibody (N-term) AW5047-U400
    Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AW5047) compared to an isotype control of rabbit IgG (blue). AW5047 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • FC - HLA-B Antibody (N-term) AW5047-U400
    Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AW5047) compared to an isotype control of rabbit IgG (blue). AW5047 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P03989
Other Accession P30490, P30488, P30487, P30486, P30485, P30483, P30481, P30479, Q04826, P18463, P30464, P30461
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 40428 Da
Additional Information
Antigen Region 62-90 aa
Other Names HLA-B; HLAB; HLA class I histocompatibility antigen, B-27 alpha chain; MHC class I antigen B*27
Dilution WB~~1:1000
FC~~1:25
Target/Specificity This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-B
Synonyms HLAB
Function Involved in the presentation of foreign antigens to the immune system.
Cellular Location Membrane; Single-pass type I membrane protein
Citations (0)

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Background

HLA-B is involved in the presentation of foreign antigens to the immune system.

References

Higgins,C.M., et.al., Ann. Rheum. Dis. 51 (7), 855-862 (1992)
Guild,B.C.et.al., J. Biol. Chem. 259 (14), 9235-9240 (1984)
Orr,H.T., et.alt., Biochemistry 18 (25), 5711-5720 (1979)

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$ 325.00
$ 110.00
Cat# AW5047-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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