|Application ||WB, IF|
|Other Accession||P70288, Q92769, P56519, Q4QQW4, O09106, P56517, Q32PJ8, O42227, Q91695, NP_004955.2|
|Reactivity||Human, Mouse, Rat|
|Predicted||Bovine, Chicken, Xenopus|
|Calculated MW||H=55;M=55;Rat=55 KDa|
|Antigen Region||70-99 aa|
|Other Names||HDAC1; RPD3L1; Histone deacetylase 1|
|Target/Specificity||This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human HDAC1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HDAC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST- mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.|
|Tissue Location||Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain|
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Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Yang, Z., et al. Clin. Chem. Lab. Med. 48(12):1785-1791(2010) Grausenburger, R., et al. J. Immunol. 185(6):3489-3497(2010) Miller, K.M., et al. Nat. Struct. Mol. Biol. 17(9):1144-1151(2010) Brandt, S., et al. Int. J. Biochem. Cell Biol. 42(9):1472-1481(2010) Leone, V., et al. Oncogene 29(30):4341-4351(2010)
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