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CTBP1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CTBP1 Antibody (C-term) AW5095-U400
    All lanes : Anti-CTBP1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Hela whole cell lysate Lane 5: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CTBP1 Antibody (C-term) AW5095-U400
    Western blot analysis of lysates from Jurkat cell line,mouse brain,rat brain tissue lysate,Raji,Hela cell line (from left to right), using CTBP1 Antibody (C-term)(Cat. #AW5095). AW5095 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • IF - CTBP1 Antibody (C-term) AW5095-U400
    Fluorescent confocal image of Hela cell stained with CTBP1 Antibody (C-term)(Cat#AW5095). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.
  • IF - CTBP1 Antibody (C-term) AW5095-U400
    Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody (C-term)(Cat#AW5095). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q13363
Other Accession NP_001319.1, NP_001012632.1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=48,46;M=48,47,40;Rat=47 KDa
Additional Information
Gene ID 1487
Antigen Region 413-440 aa
Other Names CTBP1; CTBP; C-terminal-binding protein 1
Dilution WB~~1:1000
IF~~1:10~50
Target/Specificity This CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human CTBP1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTBP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTBP1
Synonyms CTBP
Function Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed in germinal center B-cells.
Citations (0)

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Background

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

References

Roukens, M.G., et al. Nat. Cell Biol. 12(10):933-942(2010)
Choi, H.J., et al. Biochem. Biophys. Res. Commun. 400(3):396-402(2010)
Merrill, J.C., et al. J. Mol. Biol. 398(5):657-671(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)

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$ 325.00
$ 110.00
Cat# AW5095-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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