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GSTM4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GSTM4 Antibody (N-term) AW5125-U100
    Western blot analysis of lysates from MCF-7 cell line,rat lung tissue,Hela cell line (from left to right), using GSTM4 Antibody (N-term)(Cat. #AW5125). AW5125 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
  • WB - GSTM4 Antibody (N-term) AW5125-U100
    All lanes : Anti-GSTM4 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q03013
Other Accession P46439, Q9TSM4, P28161, P04905, Q9TSM5, P09488, Q9N0V4
Reactivity Human, Rat
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=26,23,19;Rat=26 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 2948
Antigen Region 16-50 aa
Other Names Glutathione S-transferase Mu 4, GST class-mu 4, GST-Mu2, GSTM4-4, GSTM4
Dilution WB~~1:2000
Target/Specificity This GSTM4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-50 amino acids from the N-terminal region of human GSTM4.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTM4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTM4
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:8203914, PubMed:8373352). Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4 (PubMed:27791009). Can also catalyzes the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions (PubMed:27791009).
Cellular Location Cytoplasm.
Tissue Location Expressed in a wide variety of tissues.
Research Areas
Citations (0)
citation

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Background

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1- chloro-2,4-dinitrobenzene.

References

Comstock K.E.,et al.J. Biol. Chem. 268:16958-16965(1993).
Zhong S.,et al.Biochem. J. 291:41-50(1993).
Ross V.L.,et al.Biochem. J. 294:373-380(1993).
Denson J.,et al.Gene 379:148-155(2006).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 370.00
Cat# AW5125-U100
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