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HLA-DRB1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HLA-DRB1 Antibody (Center) AW5127-U100
    Western blot analysis of lysates from Daudi cell line,human spleen tissue,Raji,Ramos cell line (from left to right), using HLA-DRB1 Antibody (Center)(Cat. #AW5127). AW5127 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • IHC-P - HLA-DRB1 Antibody (Center) AW5127-U100
    Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-DRB1 Antibody (Center)(Cat#AW5127). AW5127 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - HLA-DRB1 Antibody (Center) AW5127-U100
    Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-DRB1 Antibody (Center)(Cat#AW5127). AW5127 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession P04229
Other Accession Q30154
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=30 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Antigen Region 103-137 aa
Other Names HLA class II histocompatibility antigen, DRB1-1 beta chain, MHC class II antigen DRB1*1, DR-1, DR1, HLA-DRB1
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This HLA-DRB1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-137 amino acids from the Central region of human HLA-DRB1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-DRB1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)
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Background

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading.

References

Tonnelle C.,et al.EMBO J. 4:2839-2847(1985).
Bell J.I.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:3405-3409(1985).
Coppin H.L.,et al.J. Immunol. 144:984-989(1990).
Raymond C.K.,et al.Genome Res. 15:1250-1257(2005).
von Salome J.,et al.Immunogenetics 59:261-271(2007).

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Discontinued
Cat# AW5127-U100
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