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ARHGDIA Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - ARHGDIA Antibody (N-term) AW5149-U400
    Western blot analysis of lysates from A2058 cell line,mouse lung tissue,Hela,Jurkat cell line (from left to right), using ARHGDIA Antibody (N-term)(Cat. #AW5149). AW5149 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • IHC-P - ARHGDIA Antibody (N-term) AW5149-U400
    Immunohistochemical analysis of paraffin-embedded H.liver section using ARHGDIA Antibody (N-term)(Cat#AW5149). AW5149 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52565
Other Accession Q5XI73, Q99PT1, Q4R4J0, P19803
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=23;M=23;Rat=23 KDa
Additional Information
Gene ID 396
Antigen Region 25-53 aa
Other Names ARHGDIA; GDIA1; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha
Dilution WB~~1:1000
Target/Specificity This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human ARHGDIA.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARHGDIA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms GDIA1
Function Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Cellular Location Cytoplasm.
Citations (0)

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ARHGDIA belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA


Qiao,J., etc, Am. J. Physiol., Cell Physiol. 295 (5), C1161-C1168 (2008)

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$ 325.00
$ 110.00
Cat# AW5149-U400
(40 western blots)
Availability: In Stock
Bulk Size
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