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Phospho-HIST1H3B3(S10) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - Phospho-HIST1H3B3(S10) Antibody AW5153-U400
    Western blot analysis of lysate from Hela cell line, using Phospho-H3-pS10 Antibody (Cat. #AW5153). AW5153 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysate at 20ug.
  • WB - Phospho-HIST1H3B3(S10) Antibody AW5153-U400
    Western blot analysis of lysate from Hela cell line, using Phospho-H3-pS10 Antibody (Cat. #AW5153). AW5153 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysate at 20ug.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68431
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=15,M=15,Rat=15 KDa
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Antigen Region 8-35 aa
Other Names HIST1H3A; H3FA; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Dilution WB~~1:500
Target/Specificity This HIST1H3B3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-HIST1H3B3(S10) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.


Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003).
Deng, L., et al., Virology 289(2):312-326 (2001).
Deng, L., et al., Virology 277(2):278-295 (2000).
El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998).
Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).

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$ 325.00
$ 110.00
Cat# AW5153-U400
(40 western blots)
Availability: In Stock
Bulk Size
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