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PDIA6 Antibody (Center K159)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PDIA6 Antibody (Center K159) AW5167-U400
    Western blot analysis of lysates from HT-1080,K562 cell line,human liver tissue,HepG2,C6 cell line (from left to right), using PDIA6 Antibody (Center K159)(Cat. #AW5167). AW5167 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • IHC-P - PDIA6 Antibody (Center K159) AW5167-U400
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PDIA6 Antibody (Center K159) AW5167-U400
    Flow cytometric analysis of hela cells using PDIA6 Antibody (Center K159)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - PDIA6 Antibody (Center K159) AW5167-U400
    Immunohistochemical analysis of paraffin-embedded H. liver section using PDIA6 Antibody (Center K159)(Cat#AW5167). AW5167 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - PDIA6 Antibody (Center K159) AW5167-U400
    Immunohistochemical analysis of paraffin-embedded H. brain section using PDIA6 Antibody (Center K159)(Cat#AW5167). AW5167 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IF - PDIA6 Antibody (Center K159) AW5167-U400
    Fluorescent image of HepG2 cells stained with XAF1 PDIA6 Antibody (Center K159)(Cat#AW5167). AW5167 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF
Primary Accession Q15084
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=48,54,49,53;M=48;Rat=48 KDa
Additional Information
Gene ID 10130
Antigen Region 144-172 aa
Other Names PDIA6; ERP5; P5; TXNDC7; Protein disulfide-isomerase A6; Endoplasmic reticulum protein 5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7
Dilution WB~~1:1000
IHC-P~~1:100
FC~~1:10~50
IF~~1:100
Target/Specificity This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDIA6 Antibody (Center K159) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDIA6
Synonyms ERP5, P5, TXNDC7
Function May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
Cellular Location Endoplasmic reticulum lumen. Cell membrane. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545)
Tissue Location Expressed in platelets (at protein level).
Citations (0)

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Background

Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.

References

Hayano,T.,Gene 164 (2), 377-378 (1995)

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$ 325.00
$ 110.00
Cat# AW5167-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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