|Application ||WB, IHC-P|
|Other Accession||Q60HD8, Q5J7W1|
|Calculated MW||H=45;M=45;Rat=45 KDa|
|Antigen Region||305-334 aa|
|Other Names||PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2|
|Target/Specificity||This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PGK1 Antibody (Center S320) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).|
|Tissue Location||Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men|
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Provided below are standard protocols that you may find useful for product applications.
Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
Shetty, S., et al., Am. J. Respir. Cell Mol. Biol. 31(1):100-106 (2004).
Daly, E.B., et al., Biochim. Biophys. Acta 1691(1):17-22 (2004).
Daly, E.B., et al., Int. J. Biol. Markers 19(2):170-172 (2004).
Saito, Y., et al., Biochem. Biophys. Res. Commun. 314(2):396-402 (2004).
Krishnan, P., et al., J. Biol. Chem. 278(38):36726-36732 (2003).
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