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SSPN Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SSPN Antibody (N-term) AW5200-U400
    Western blot analysis of lysates from HL-60,K562,MDA-MB-453 cell line (from left to right), using SSPN Antibody (N-term)(Cat. #AW5200). AW5200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q14714
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=27,14 KDa
Additional Information
Antigen Region 6-34 aa
Other Names SSPN;KRAG; Sarcospan; Sarcospan; K-ras oncogene-associated protein; Sarcospan; Kirsten-ras-associated protein
Dilution WB~~1:1000
Target/Specificity This SSPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human SSPN.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSSPN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SSPN
Synonyms KRAG
Function Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein Note=Also found in myotendinous junctions and in the postsynaptic membrane of neuromuscular junctions.
Tissue Location Isoform 1 is expressed exclusively in heart and skeletal muscle. Isoform 2 is expressed in heart, skeletal muscle, thymus, prostate, testis, ovary, small intestine, colon and spleen EMBL; AF016028; AAC61660.2; -; mRNA EMBL; AL136756; CAB66690.1; -; mRNA EMBL; AK092925; BAG52629.1; -; mRNA EMBL; AC022509; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC055720; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC062299; AAH62299.1; -; mRNA EMBL; X89105; CAA61479.1; -; mRNA CCDS; CCDS44850.1; -. [Q14714-3] CCDS; CCDS8707.1; -. [Q14714-1] PIR; S57712; S57712 RefSeq; NP_001129295.1; NM_001135823.1. [Q14714-3] RefSeq; NP_005077.2; NM_005086.4. [Q14714-1] RefSeq; XP_011519155.1; XM_011520853.2. [Q14714-3] RefSeq; XP_011519156.1; XM_011520854.2. [Q14714-3] RefSeq; XP_011519157.1; XM_011520855.2. [Q14714-3] UniGene; Hs.183428; - UniGene; Hs.736914; - ProteinModelPortal; Q14714; - BioGrid; 113755; 1 MINT; MINT-4721894; - STRING; 9606.ENSP00000242729; - iPTMnet; Q14714; - PhosphoSitePlus; Q14714; - SwissPalm; Q14714; - BioMuta; SSPN; - DMDM; 13431900; - PaxDb; Q14714; - PeptideAtlas; Q14714; - PRIDE; Q14714; - DNASU; 8082; - Ensembl; ENST00000242729; ENSP00000242729; ENSG00000123096. [Q14714-1] Ensembl; ENST00000422622; ENSP00000396087; ENSG00000123096. [Q14714-3] Ensembl; ENST00000540266; ENSP00000442893; ENSG00000123096. [Q14714-3] GeneID; 8082; - KEGG; hsa:8082; - UCSC; uc001rhd.4; human. [Q14714-1] CTD; 8082; - DisGeNET; 8082; - GeneCards; SSPN; - HGNC; HGNC:11322; SSPN MIM; 601599; gene neXtProt; NX_Q14714; - OpenTargets; ENSG00000123096; - PharmGKB; PA36146; - eggNOG; ENOG410IK33; Eukaryota eggNOG; ENOG4111WM7; LUCA GeneTree; ENSGT00390000007747; - HOGENOM; HOG000065718; - HOVERGEN; HBG005016; - InParanoid; Q14714; - OMA; AGIIVCV; - OrthoDB; EOG091G0MQZ; - PhylomeDB; Q14714; - TreeFam; TF332100; - BioCyc; ZFISH:ENSG00000123096-MONOMER; - GenomeRNAi; 8082; - PRO; PR:Q14714; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000123096; - CleanEx; HS_SSPN; - ExpressionAtlas; Q14714; baseline and differential Genevisible; Q14714; HS GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW GO; GO:0016010; C:dystrophin-associated glycoprotein complex; TAS:ProtInc GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell GO; GO:0042383; C:sarcolemma; IEA:UniProtKB-SubCell GO; GO:0030133; C:transport vesicle; IDA:LIFEdb GO; GO:0007155; P:cell adhesion; TAS:ProtInc GO; GO:0006936; P:muscle contraction; TAS:ProtInc InterPro; IPR007237; CD20-like InterPro; IPR030429; Sarcospan PANTHER; PTHR15260; PTHR15260; 1 Pfam; PF04103; CD20; 1 2: Evidence at transcript level; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Membrane; Polymorphism; Postsynaptic cell membrane; Reference proteome; Synapse; Transmembrane; Transmembrane helix CHAIN 1 243 Sarcospan /FTId=PRO_0000072226 TOPO_DOM 1 53 Cytoplasmic. TRANSMEM 54 74 Helical. TOPO_DOM 75 86 Extracellular. TRANSMEM 87 107 Helical. TOPO_DOM 108 122 Cytoplasmic. TRANSMEM 123 143 Helical. TOPO_DOM 144 193 Extracellular. TRANSMEM 194 214 Helical. TOPO_DOM 215 243 Cytoplasmic. VAR_SEQ 1 103 Missing (in isoform 3) /FTId=VSP_046351 VAR_SEQ 108 218 Missing (in isoform 2) /FTId=VSP_004431 VARIANT 186 186 S -> N (in dbSNP:rs12313670) /FTId=VAR_051384 VARIANT 228 228 V -> I (in dbSNP:rs12313736) /FTId=VAR_051385 CONFLICT 13 13 Missing (in Ref. 1). SEQUENCE 243 AA; 26618 MW; 0D6B24B9848253F9 CRC64; MGKNKQPRGQ QRQGGPPAAD AAGPDDMEPK KGTGAPKECG EEEPRTCCGC RFPLLLALLQ LALGIAVTVV GFLMASISSS LLVRDTPFWA GIIVCLVAYL GLFMLCVSYQ VDERTCIQFS MKLLYFLLSA LGLTVCVLAV AFAAHHYSQL TQFTCETTLD SCQCKLPSSE PLSRTFVYRD VTDCTSVTGT FKLFLLIQMI LNLVCGLVCL LACFVMWKHR YQVFYVGVRI CSLTASEGPQ QKI
Citations (0)

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Background

Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.

References

Crosbie R.H., et al. J. Biol. Chem. 272:31221-31224(1997).
Crosbie R.H., et al. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Wiemann S., et al. Genome Res. 11:422-435(2001).
Heighway J., et al. Genomics 35:207-214(1996).

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$ 325.00
$ 110.00
Cat# AW5200-U400
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(40 western blots)
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