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CYB5R3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CYB5R3 Antibody (Center) AW5217-U100
    Western blot analysis of lysates from Hela,HepG2,A431 cell line,rat liver tissue lysate(from left to right), using CYB5R3(soluble form) Antibody (Center)(Cat. #AW5217). AW5217 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P00387
Other Accession P20070, P83686, Q9DCN2, Q60HG4, P07514
Reactivity Human, Rat
Predicted Bovine, Monkey, Mouse, Pig
Host Rabbit
Clonality polyclonal
Calculated MW H=34,32,38;M=34,32;Rat=34,35,32 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 1727
Antigen Region 111-144 aa
Other Names NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1
Dilution WB~~1:1000
Target/Specificity This CYB5R3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-144 amino acids from the Central region of human CYB5R3
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R3 (HGNC:2873)
Synonyms DIA1
Function Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}
Tissue Location [Isoform 2]: Expressed at late stages of erythroid maturation.
Research Areas
Citations (0)
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Background

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

References

Tomatsu S.,et al.Gene 80:353-361(1989).
Voice M.W.,et al.Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
Yoon B.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Lan F.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.

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$ 370.00
Cat# AW5217-U100
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