|Application ||IHC-P, IF, FC, WB|
|Other Accession||P10719, P56480, P00829|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||H=57 KDa|
|Antigen Region||135-163 aa|
|Other Names||ATP5B; ATPMB; ATPSB; ATP synthase subunit beta, mitochondrial|
|Target/Specificity||This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP5B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.|
|Cellular Location||Mitochondrion. Mitochondrion inner membrane. Note=Peripheral membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.
Neckelmann,N., et.al., Genomics 5 (4), 829-843 (1989) Ohta,S., et.al., J. Biol. Chem. 263 (23), 11257-11262 (1988) Wallace,D.C., et.al., Curr. Genet. 12 (2), 81-90 (1987)
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