|Application ||FC, IHC-P, WB, IF|
|Calculated MW||H=37,44 KDa|
|Antigen Region||317-343 aa|
|Other Names||CD46; MCP; MIC10; Membrane cofactor protein; TLX; Trophoblast leukocyte common antigen; CD_antigen=CD46; Flags: Precursor|
|Target/Specificity||This CD46 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-343 amino acids from the C-terminal region of human CD46.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD46 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.|
|Cellular Location||Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single- pass type I membrane protein Note=Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide|
|Tissue Location||Expressed by all cells except erythrocytes.|
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Provided below are standard protocols that you may find useful for product applications.
CD46 is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. This gene is found in a cluster on chromosome 1q32 with other genes encoding structural components of the complement system.
Weyand, N.J., et al. J. Immunol. 184(2):694-701(2010)
Santiago, C., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 1), 91-94 (2010)
Sullivan, M., et al. Ann. Hum. Genet. 74(1):17-26(2010)
Lee, S.W., et al. J. Cell Biol. 156(6):951-957(2002)
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