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GPI Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - GPI Antibody (C-term) AW5240-U400
    Western blot analysis of lysates from rat liver tissue lysate,Ramos cell line (from left to right), using GPI Antibody (C-term)(Cat. #AW5240). AW5240 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • FC - GPI Antibody (C-term) AW5240-U400
    GPI Antibody (C-term) (Cat. #AW5240) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06744
Other Accession Q4R591
Reactivity Human, Rat
Predicted Mouse, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=63,64;M=63;Rat=63 KDa
Additional Information
Gene ID 2821
Antigen Region 445-473 aa
Other Names GPI; Glucose-6-phosphate isomerase; Autocrine motility factor; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36
Dilution WB~~1:1000
Target/Specificity This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPI
Function Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Cellular Location Cytoplasm. Secreted
Citations (0)

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GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.


Shih, W.L., et al. Cancer Lett. 290(2):223-237(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Araki, K., et al. J. Biol. Chem. 284(47):32305-32311(2009)
Tsutsumi, S., et al. Int. J. Oncol. 35(5):1117-1121(2009)
Funasaka, T., et al. Cancer Res. 69(13):5349-5356(2009)
Yanagawa, T., et al. J. Biol. Chem. 280(11):10419-10426(2005)
Haga, A., et al. Biochim. Biophys. Acta 1480 (1-2), 235-244 (2000)

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$ 325.00
$ 110.00
Cat# AW5240-U400
(40 western blots)
Availability: In Stock
Bulk Size
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