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HSP40 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - HSP40 Antibody AW5253-U400
    Western blot analysis of lysates from Hela,mouse NIH/3T3 cell line (from left to right), using HSP40 Antibody(Cat. #AW5253). AW5253 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25685
Other Accession NP_006136
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=38;M=38 KDa
Additional Information
Gene ID 3337
Antigen Region Full length aa
Other Names DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; Human DnaJ protein 1
Dilution WB~~ 1:1000
Target/Specificity This HSP40 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human HSP40.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP40 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms DNAJ1, HDJ1, HSPF1
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Cellular Location Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock
Citations (0)

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DnaJ (Hsp40) belongs to the DnaJ-class of molecular chaperones with a C-terminal Zn finger domain. HSP40 (DnaJ) together with DnaK and GrpE form a molecular chaperone that is involved in formation of protein complexes, protein folding, prevention of protein aggregation, and protein turnover and export. Several human neurodegenerative diseases involve the expansion of a polyglutamine within the disease proteins. Molecular chaperones such as HSP40 complexes can modulate polyglutamine pathogenesis In transgenic Drosophila disease models of Machado-Joseph disease and Huntington disease Hdj1, the Drosophila homolog to human HSP40, demonstrates substrate specificity for polyglutamine proteins suppression in combination with other molecular chapterones of neurotoxicity, and altered solubility of mutant polyglutamine proteins.


Ohtsuka, K., et al., Cell Stress Chaperones 5(2):98-112 (2000). Hata, M., et al., Biochim. Biophys. Acta 1397(1):43-55 (1998). Hata, M., et al., Genomics 38(3):446-449 (1996). Ohtsuka, K., Biochem. Biophys. Res. Commun. 197(1):235-240 (1993).

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$ 325.00
$ 110.00
Cat# AW5253-U400
(40 western blots)
Availability: In Stock
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