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WEE1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - WEE1 Antibody (Center) AW5256-U400
    Western blot analysis of lysates from Jurkat,A431 cell line (from left to right), using WEE1 Antibody (A159)(Cat. #AW5256). AW5256 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30291
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=72 KDa
Additional Information
Gene ID 7465
Antigen Region 144-173 aa
Other Names WEE1; Wee1-like protein kinase; Wee1A kinase
Dilution WB~~ 1:1000
Target/Specificity This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the Central region of human WEE1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWEE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WEE1
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Cellular Location Nucleus.
Citations (0)

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WEE1 is a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.


Kawasaki, H., et al., Oncogene 22(44):6839-6844 (2003).
Hashimoto, O., et al., Mol. Carcinog. 36(4):171-182 (2003).
Yuan, H., et al., J. Virol. 77(3):2063-2070 (2003).
Masaki, T., et al., Hepatology 37(3):534-543 (2003).
de Noronha, C.M., et al., Science 294(5544):1105-1108 (2001).

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$ 325.00
$ 110.00
Cat# AW5256-U400
(40 western blots)
Availability: In Stock
Bulk Size
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