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CAPN2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • WB - CAPN2 Antibody AW5279-U400
    Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody(cat. #AW5279). AW5279 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • FC - CAPN2 Antibody AW5279-U400
    Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, Cat#AW5279) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • IHC-P - CAPN2 Antibody AW5279-U400
    Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody(Cat#AW5279). AW5279 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17655
Reactivity Human, Rat
Predicted Mouse
Host Mouse
Clonality monoclonal
Calculated MW H=80,71;M=80,71 KDa
Additional Information
Gene ID 824
Other Names Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
Dilution WB~~1:1000
Target/Specificity This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPN2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAPN2
Synonyms CANPL2
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).
Cellular Location Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding
Tissue Location Ubiquitous.
Citations (0)

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Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.


Imajoh S.,et al.Biochemistry 27:8122-8128(1988).
Ye Z.,et al.Biochem. Biophys. Res. Commun. 275:223-227(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Hata A.,et al.J. Biol. Chem. 264:6404-6411(1989).

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$ 325.00
$ 110.00
Cat# AW5279-U400
(40 western blots)
Availability: In Stock
Bulk Size
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