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SMAD6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - SMAD6 Antibody (Center) AW5282-U400
    Western blot analysis of lysates from HepG2,HT-29 cell line (from left to right), using SMAD6Antibody(Center)(Cat. #AW5282). AW5282 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • FC - SMAD6 Antibody (Center) AW5282-U400
    SMAD6 Antibody (Center) (Cat. #AW5282) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43541
Other Accession O35182, NP_005576.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=53,26,35;M=54 KDa
Additional Information
Gene ID 4091
Antigen Region 358-386 aa
Other Names SMAD6; MADH6; Mothers against decapentaplegic homolog 6; SMAD family member 6
Dilution WB~~1:1000
Target/Specificity This SMAD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-386 amino acids from the Central region of human SMAD6.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMAD6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD6
Synonyms MADH6
Function Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.
Cellular Location Nucleus.
Tissue Location Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue
Research Areas
Citations (0)

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SMAD6 belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling.


Tseng, Z.H., et al. Heart Rhythm 6(12):1745-1750(2009)
Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009)
Yu, H., et al. Acta Derm. Venereol. 89(4):351-356(2009)

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$ 325.00
$ 110.00
Cat# AW5282-U400
(40 western blots)
Availability: In Stock
Bulk Size
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