|Application ||WB, IHC-P|
|Other Accession||Q9WVH4, NP_963853.1, NP_001446.1|
|Calculated MW||H=71 KDa|
|Antigen Region||1-30 aa|
|Other Names||FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1|
|Target/Specificity||This FOXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXO3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FOXO3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' (PubMed:21329882). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301).|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Mitochondrion matrix Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Retention in the cytoplasm contributes to its inactivation (PubMed:10102273, PubMed:15084260, PubMed:16751106). Translocates to the nucleus upon oxidative stress and in the absence of survival factors (PubMed:10102273, PubMed:16751106). Translocates in a AMPK- dependent manner into the mitochondrion in response to metabolic stress (PubMed:23283301, PubMed:29445193)|
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Provided below are standard protocols that you may find useful for product applications.
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Zhuo de, X., et al. J. Biol. Chem. 285(41):31491-31501(2010)
Tudzarova, S., et al. EMBO J. 29(19):3381-3394(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Mikse, O.R., et al. Cancer Res. 70(15):6205-6215(2010)
Chen, J., et al. PLoS ONE 5 (8), E12293 (2010) :
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