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TBP Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - TBP Antibody AW5294-U400
    Western blot analysis of lysates from Hela,HepG2,mouse NIH/3T3,Jurkat cell line and human testis tissue (from left to right), using TBP Antibody (Cat. #AW5294). AW5294 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • IHC-P - TBP Antibody AW5294-U400
    Immunohistochemical analysis of paraffin-embedded H. testis section using TBP Antibody(Cat#AW5294). AW5294 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P20226
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Calculated MW H=38;M=35 KDa
Additional Information
Gene ID 6908
Antigen Region 1-308 aa
Other Names TBP;GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA-box-binding protein; TATA sequence-binding protein; TATA-box-binding protein; TATA-binding factor; TATA-box-binding protein; TATA-box factor; TATA-box-binding protein; Transcription initiation factor TFIID TBP subunit
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity Purified His-tagged TBP protein was used to produced this monoclonal antibody.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBP
Synonyms GTF2D1, TF2D, TFIID {ECO:0000303|PubMed:
Function General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Cellular Location Nucleus.
Tissue Location Widely expressed, with levels highest in the testis and ovary.
Citations (0)

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Background

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

References

Hoffmann A., et al. Nature 346:387-390(1990).
Peterson M.G., et al. Science 248:1625-1630(1990).
Kao C.C., et al. Science 248:1646-1650(1990).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Mungall A.J., et al. Nature 425:805-811(2003).

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$ 325.00
$ 110.00
Cat# AW5294-U400
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Quantity:
(40 western blots)
Availability: In Stock
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