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COX6A2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - COX6A2 Antibody (Center) AW5300-U400
    Western blot analysis of lysates from human skeletal muscle and huamn heart tissue lysate (from left to right), using COX6A2 Antibody (Center)(Cat. #AW5300). AW5300 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • IHC-P - COX6A2 Antibody (Center) AW5300-U400
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using COX6A2 Antibody (Center)(Cat#AW5300). AW5300 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q02221
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=11 KDa
Additional Information
Gene ID 1339
Antigen Region 37-66 aa
Other Names COX6A2; COX6A; COX6AH; Cytochrome c oxidase subunit 6A2, mitochondrial; Cytochrome c oxidase polypeptide VIa-heart; Cytochrome c oxidase subunit VIA-muscle
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This COX6A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human COX6A2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOX6A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COX6A2
Synonyms COX6A, COX6AH
Function This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Cellular Location Mitochondrion inner membrane.
Tissue Location Expressed specifically in heart and muscle.
Citations (0)

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Background

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.

References

Bachman, N.J., et al. Genomics 42(1):146-151(1997)
Lanfranchi, G., et al. Genome Res. 6(1):35-42(1996)

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$ 325.00
$ 110.00
Cat# AW5300-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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