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STOM Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - STOM Antibody (N-term) AW5370-U400
    All lanes : Anti-STOM Antibody (N-term) at 1/1000 dilution Lane 1: human lung lysates Lane 2: Hela whole cell lysates Lane 3: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P27105
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=32,13 KDa
Additional Information
Gene ID 2040
Antigen Region 2-36 aa
Other Names Erythrocyte band 7 integral membrane protein, Protein 72b, Stomatin, STOM, BND7, EPB72
Dilution WB~~1:1000
Target/Specificity This STOM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human STOM.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTOM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STOM
Synonyms BND7, EPB72
Function Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane raft Melanosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P54116}. Note=Localizes to juxtanuclear structure probably derived from the Golgi apparatus (PubMed:9243190). Colocalizes with cortical actin microfilaments at small plasma membrane protrusions (PubMed:9243190). Associates with alpha-granular lipid rafts (PubMed:12130500). Translocates from the alpha-granular lipid rafts to the cell membrane on thrombin activation and selectively enriched in released microvesicles (PubMed:12130500). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Tissue Location Detected in erythrocytes (at protein level). Widely expressed.
Citations (0)

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Background

Thought to regulate cation conductance. May regulate ASIC2 and ASIC3 gating (By similarity).

References

Hiebl-Dirschmied C.M.,et al.Biochim. Biophys. Acta 1090:123-124(1991).
Stewart G.W.,et al.Blood 79:1593-1601(1992).
Unfried I.,et al.Genomics 30:521-528(1995).
Gallagher P.G.,et al.J. Biol. Chem. 270:26358-26363(1995).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 325.00
$ 110.00
Cat# AW5370-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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