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DAPK2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - DAPK2 Antibody (N-term) AW5377-U400
    All lanes : Anti-DAPK2 Antibody M1 at 1:1000 dilution Lane 1: mouse brain lysates Lane 2: SH-SY5Y whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UIK4
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=43,56;M=43,56 KDa
Additional Information
Gene ID 23604
Antigen Region 1-30 aa
Other Names Death-associated protein kinase 2, DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2
Dilution WB~~1:1000
Target/Specificity This DAPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DAPK2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDAPK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAPK2
Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation.
Cellular Location Cytoplasm. Cytoplasmic vesicle, autophagosome lumen
Tissue Location Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages
Citations (0)

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DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.


Satoh, A., et al., Br. J. Cancer 86(11):1817-1823 (2002).
Chan, M.W., et al., Clin. Cancer Res. 8(2):464-470 (2002).
Wong, T.S., et al., Clin. Cancer Res. 8(2):433-437 (2002).
Shani, G., et al., EMBO J. 20(5):1099-1113 (2001).
Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).

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$ 325.00
$ 110.00
Cat# AW5377-U400
(40 western blots)
Availability: In Stock
Bulk Size
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