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GRB2 Antibody (Y209)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - GRB2 Antibody (Y209) AW5382-U400
    All lanes : Anti-GRB2 Antibody pY209 at 1:1000 dilution Lane 1: Ramos whole cell lysates Lane 2: PC-12 whole cell lysates Lane 3: A431 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62993
Other Accession P62994, Q60631, Q07883, Q6GPJ9, P87379
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=25,21;M=25,21;R=25 KDa
Additional Information
Gene ID 2885
Antigen Region 187-216 aa
Other Names Growth factor receptor-bound protein 2, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2, GRB2, ASH
Dilution WB~~1:1000
Target/Specificity This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from human GRB2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRB2 Antibody (Y209) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRB2
Synonyms ASH
Function Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Cellular Location Nucleus. Cytoplasm. Endosome. Golgi apparatus
Citations (0)

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GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.


Kondo,A., J. Biol. Chem. 283 (3), 1428-1436 (2008) Morimatsu,M., Proc. Natl. Acad. Sci. U.S.A. 104 (46), 18013-18018 (2007) Martinez,N., Cell. Signal. 19 (11), 2277-2285 (2007)

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$ 325.00
$ 110.00
Cat# AW5382-U400
(40 western blots)
Availability: In Stock
Bulk Size
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