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SEMA4F Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SEMA4F Antibody (C-term) AW5403-U100
    All lanes : Anti-SEMA4F Antibody (C-term) at 1:1000 dilution Lane 1: rat lung lysates Lane 2: U87-MG whole cell lysates Lane 3: HepG2 whole cell lysates Lane 4: A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O95754
Other Accession Q9Z143, Q9Z123
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=84,66;M=84;R=84 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 10505
Antigen Region 695-709 aa
Other Names Semaphorin-4F, Semaphorin-M, Sema M, Semaphorin-W, Sema W, SEMA4F, SEMAM, SEMAW
Dilution WB~~1:1000
Target/Specificity This SEMA4F antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 695-709 amino acids from the C-terminal region of human SEMA4F.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEMA4F Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SEMA4F
Synonyms SEMAM, SEMAW
Function Probable cell surface receptor that regulates oligodendroglial precursor cell migration (By similarity). Might also regulate differentiation of oligodendroglial precursor cells (By similarity). Has growth cone collapse activity against retinal ganglion-cell axons (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Postsynaptic density {ECO:0000250|UniProtKB:Q9Z143}. Perikaryon {ECO:0000250|UniProtKB:Q9Z123}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q9Z123}. Note=Colocalizes with DLG4 at synapses {ECO:0000250|UniProtKB:Q9Z123}
Citations (0)
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Background

Has growth cone collapse activity against retinal ganglion-cell axons (By similarity).

References

Encinas J.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:2491-2496(1999).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Hillier L.W.,et al.Nature 434:724-731(2005).
Jang W.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.

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$ 370.00
Cat# AW5403-U100
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