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CTDSP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CTDSP1 Antibody (Center) AW5411-U400
    All lanes : Anti-CTDSP1 Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9GZU7
Other Accession P58466
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=29;M=29 KDa
Additional Information
Gene ID 58190
Antigen Region 96-130 aa
Other Names Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF, SCP1
Dilution WB~~1:1000
Target/Specificity This CTDSP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-130 amino acids from the Central region of human CTDSP1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTDSP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTDSP1
Synonyms NIF3, NLIIF, SCP1
Function Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Cellular Location Nucleus. Note=Colocalizes with RNA polymerase II
Tissue Location Expression is restricted to non-neuronal tissues. Highest expression in skeletal muscle, spleen, lung and placenta.
Citations (0)

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Background

Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.

References

Marquet S.,et al.Mamm. Genome 11:755-762(2000).
Yeo M.,et al.J. Biol. Chem. 278:26078-26085(2003).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Yeo M.,et al.Science 307:596-600(2005).

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$ 325.00
$ 110.00
Cat# AW5411-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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