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WDR5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - WDR5 Antibody (C-term) AW5416-U400
    All lanes : Anti-WDR5 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P61964
Other Accession Q9V3J8, Q498M4, P61965, Q2KIG2
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=37;M=37;R=37 KDa
Additional Information
Gene ID 11091
Antigen Region 321-354 aa
Other Names WD repeat-containing protein 5, BMP2-induced 3-kb gene protein, WDR5, BIG3
Dilution WB~~1:1000
Target/Specificity This WDR5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 321-354 amino acids from the C-terminal region of human WDR5.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR5
Synonyms BIG3
Function Contributes to histone modification. May position the N- terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
Cellular Location Nucleus
Citations (0)

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Background

Contributes to histone modification. May position the N- terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.

References

Young J.M.,et al.Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Wysocka J.,et al.Genes Dev. 17:896-911(2003).
Hughes C.M.,et al.Mol. Cell 13:587-597(2004).
Yokoyama A.,et al.Mol. Cell. Biol. 24:5639-5649(2004).

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$ 325.00
$ 110.00
Cat# AW5416-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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