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PIP4K2A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - PIP4K2A Antibody (C-term) AW5494-U400
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling PIP4K2A with AW5494 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
  • IHC-P - PIP4K2A Antibody (C-term) AW5494-U400
    Immunohistochemical analysis of paraffin-embedded H. brain section using PIP4K2A Antibody (C-term)(Cat#AW5494). AW5492 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • FC - PIP4K2A Antibody (C-term) AW5494-U400
    Flow cytometric analysis of Hela cells using PIP4K2A Antibody (C-term)(green, Cat#AW5494) compared to an isotype control of rabbit IgG(blue). AW5492 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - PIP4K2A Antibody (C-term) AW5494-U400
    All lanes : Anti-PIP4K2A Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysates Lane 2: Hela whole cell lysates Lane 3: K562 whole cell lysates Lane 4: mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, IF
Primary Accession P48426
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=46, 40;M=46;R=46 KDa
Additional Information
Gene ID 5305
Antigen Region 329-363 aa
Other Names Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha, Diphosphoinositide kinase 2-alpha, PIP5KIII, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, PI(5)P 4-kinase type II alpha, PIP4KII-alpha, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PtdIns(5)P-4-kinase isoform 2-alpha, PIP4K2A, PIP5K2, PIP5K2A
Dilution IF~~1:25
IHC-P~~1:25
FC~~1:25
WB~~1:1000
Target/Specificity This PIP4K2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 329-363 amino acids from the C-terminal region of human PIP4K2A.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIP4K2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIP4K2A
Synonyms PIP5K2, PIP5K2A
Function Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.
Cellular Location Cell membrane. Nucleus. Cytoplasm. Note=May translocate from the cytosol to the cell membrane upon activation of tyrosine phosphorylation. May translocate from the inner to the outer segments of the rod photoreceptor cells in response to light (By similarity). Localization to the nucleus is modulated by the interaction with PIP4K2B.
Tissue Location Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
Citations (0)

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Background

Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

References

Boronenkov I.V.,et al.J. Biol. Chem. 270:2881-2884(1995).
Boronenkov I.V.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Divecha N.,et al.Biochem. J. 309:715-719(1995).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 325.00
$ 110.00
Cat# AW5494-U400
Size:
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(40 western blots)
Availability: In Stock
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