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DKK1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - DKK1 Antibody AW5504-U200
    AW5504 staining DKK1 in Human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • FC - DKK1 Antibody AW5504-U200
    Overlay histogram showing U-2 OS cells stained with AW5504 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5504, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - DKK1 Antibody AW5504-U200
    All lanes : Anti-DKK1 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P
Primary Accession O94907
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=29;M=29 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 22943
Antigen Region Recombinant protein of full sequence aa
Other Names Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1
Dilution IHC-P~~1:25
FC~~1:25
WB~~1:2000
Target/Specificity This DKK1 antibody is generated from a rabbit immunized with a recombinant protein of human DKK1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDKK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DKK1
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Cellular Location Secreted.
Tissue Location Placenta.
Research Areas
Citations ( 0 )

Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

References

Fedi P.,et al.J. Biol. Chem. 274:19465-19472(1999).
Krupnik V.E.,et al.Gene 238:301-313(1999).
Tate G.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Roessler E.,et al.Cytogenet. Cell Genet. 89:220-224(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

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$ 325.00
$ 110.00
Cat# AW5504-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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